Bash

/home/biol/perl5:是一個目錄

  • November 1, 2020

以下腳本崩潰:

#!/bin/bash
#usage: sh oxford_pbs.sh ref.fa AA.fasta hybrid.gff3

cov=50
ide=100


for ((i=70;i<=${ide};i++));
do
 input=$(basename $(echo ${2} | sed 's|.gff3||g'))

 #cat <<EOF
 qsub <<EOF
#!/bin/bash -l

#PBS -N filter
#PBS -l walltime=20:00:00
#PBS -j oe
#PBS -l select=1:ncpus=1:mem=30G
#PBS -M m.lorenc@qut.edu.au
##PBS -m bea

cd \$PBS_O_WORKDIR
module load bioperl/1.7.1-foss-2017a r/3.4.2-bioconductor-foss-2017a
eval "$(perl -I$HOME/perl5/lib/perl5 -Mlocal::lib)"

agat_sp_extract_sequences.pl --gff ${2}-ide${i}-cov${cov}-best-hit.gff3 -f ${1} -p -o ${2}-ide${i}-cov${cov}-best-hit.AA.fasta
EOF
done

出現此錯誤:

/var/spool/PBS/mom_priv/jobs/8352088.pbs.SC: line 24: /home/biol/perl5: Is a directory
/var/spool/PBS/mom_priv/jobs/8352088.pbs.SC: line 27: agat_sp_extract_sequences.pl: command not found

但是,這兩個命令在命令行上執行沒有任何問題:

> module load bioperl/1.7.1-foss-2017a r/3.4.2-bioconductor-foss-2017a
> eval "$(perl -I$HOME/perl5/lib/perl5 -Mlocal::lib)"
> 
> agat_sp_extract_sequences.pl --help

------------------------------------------------------------------------------
|   Another GFF Analysis Toolkit (AGAT) - Version: v0.1.0                      |
|   https://github.com/NBISweden/AGAT                                          |
|   National Bioinformatics Infrastructure Sweden (NBIS) - www.nbis.se         |
------------------------------------------------------------------------------

為什麼 Perl 腳本不在 Bash 腳本中執行?

先感謝您

問題是$(perl ...)命令替換將立即完成,因為您已經擴展qsub <<EOF而不是qsub <<'EOF'為了${i}擴展。

perl 將輸出可以包含空格的行,例如

PERL_MB_OPT="--install_base \"/home/user/perl5\""

但是當你在最終腳本的執行中評估這些時,它會變成

PERL_MB_OPT=--install_base "/home/user/perl5"

你會錯誤/home/user/perl5是一個目錄。最簡單的解決方案是將 perl 推遲到以後:

eval "\$(perl -I$HOME/perl5/lib/perl5 -Mlocal::lib)"

引用自:https://unix.stackexchange.com/questions/617404